lambda dna midi prep kit Search Results


lambda ladder pfge marker kit  (New England Biolabs)
96
New England Biolabs lambda ladder pfge marker kit
Lambda Ladder Pfge Marker Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ds dna kits thermo fisher scientific  (Thermo Fisher)
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Thermo Fisher ds dna kits thermo fisher scientific
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
Ds Dna Kits Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epitect bisulfite kit  (Qiagen)
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Qiagen epitect bisulfite kit
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
Epitect Bisulfite Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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enzymatic methyl seq kit  (New England Biolabs)
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New England Biolabs enzymatic methyl seq kit
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
Enzymatic Methyl Seq Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phase lock gel kit  (5 PRIME)
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5 PRIME phase lock gel kit
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
Phase Lock Gel Kit, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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assays nebnext ultra ii dna library prep kit for illumina neb  (New England Biolabs)
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New England Biolabs assays nebnext ultra ii dna library prep kit for illumina neb
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
Assays Nebnext Ultra Ii Dna Library Prep Kit For Illumina Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d7001 qubit dna hs assay kit invitrogen  (Zymo Research)
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Zymo Research d7001 qubit dna hs assay kit invitrogen
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
D7001 Qubit Dna Hs Assay Kit Invitrogen, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t1030s nebnext q5u master mix neb  (New England Biolabs)
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New England Biolabs t1030s nebnext q5u master mix neb
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
T1030s Nebnext Q5u Master Mix Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard lambda dna preparation kit  (Promega)
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Promega wizard lambda dna preparation kit
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
Wizard Lambda Dna Preparation Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr ssp kits  (Thermo Fisher)
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Thermo Fisher pcr ssp kits
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
Pcr Ssp Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e0554 qiaquick pcr purification kit qiagen  (Qiagen)
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Qiagen e0554 qiaquick pcr purification kit qiagen
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
E0554 Qiaquick Pcr Purification Kit Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e7335l nebnext rna library prep kit new england biolabs  (New England Biolabs)
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New England Biolabs e7335l nebnext rna library prep kit new england biolabs
(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated <t>DNA</t> complexes and (c) total double stranded <t>DNA</t> <t>quantitation</t> following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
E7335l Nebnext Rna Library Prep Kit New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated DNA complexes and (c) total double stranded DNA quantitation following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Neutrophil extracellular trap fragments stimulate innate immune responses that prevent lung transplant tolerance

doi: 10.1111/ajt.15163

Figure Lengend Snippet: (a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated DNA complexes and (c) total double stranded DNA quantitation following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.

Article Snippet: NETs release in culture and plasma was quantified with Quanti-IT Pico Green double stranded (ds) DNA kits (Thermo Fisher Scientific) using lambda DNA as quantitation standards in accordance with manufacturers recommendations.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Saline, Standard Deviation